NormaladultkeratinocytesinKGM-Gold™BulletKit™seenunderlightmicroscopy
ProductOverview:
| CryopreservedNormalHumanEpidermalKeratinocytes areguaranteedthrough 18populationdoublingsforadultcellsand20populationdoublingsforneonatalcells.Allcells testnegativeformycoplasma,bacteria,yeast,andfungi.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased. | Interestedtolearnmore?
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MostofLonza"sprimarycellsarealsoavailableas proliferatingcells ineitherflasksorplates,oraspelletsinRNALater®.
DiseasedHumanEpidermalKeratinocytes(D-HEK) areisolatedfromhumandonorsthathavebeendiagnosedwithdiabetestypeIIdisease.Furtherdonorinformation,includingmedications,BMI,anddiseasedurationmaybeobtainedbycontactingLonzaScientificSupport. DiseasedcellsaregrowninthesameoptimizedmediasystemasNormalHumanKeratinocytes.
RecommendedMedium:KGM-Gold™BulletKit™ RecommendedSubculturingReagents: ReagentPack™SubcultureReagents | WhitePaper LearnwhyKGM-Gold™Mediumoffersoptimalcultureconditionsforhumanprimarykeratinocytes
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SelectReferenceswithLonza’sKeratinocytes:
1)Protectivebarrier:Keratinocytes werestimulatedwithhistamineorhistaminereceptorligandstoshowtheformationofadefectiveskinbarrier. GschwandtnerM,MildnerM, MlitzV, GruberF, EckhartL,WerfelT,GutzmerR,EliasPMandTschachlerE. Histaminesuppressesepidermalkeratinocytedifferentiationandimpairsskinbarrierfunctioninahumanskinmodel.EuropeanJournalofAllergyandClinicalimmunology(2013);68(1):37–47.
2)Skinmodel:Neonatalkeratinocyteswereculturedinamicrofluidicsystemtopreciselyexaminethemorphology,behaviorandviABIlityofindividualcells. AdrianT.O’Neill,NancyA.Monteiro-Riviere,andGlennM.Walker.Characterizationofmicrofluidichumanepidermalkeratinocyteculture.Cytotechnology(2008);56(3):197–207.
3)Cancer:KeratinocyteswereusedinthisstudytodemonstratethatdysregulatedactivationoftypeIinterferoninnateimmunityisimplicatedinthemolecularprocessestriggeredbyanti-EGFRdrugsandleADIngtopersistentskininflammation. CarrieHS,HongY,YunboL,JenniferSM,SrideviB,GayleneS,JudithACandThomasRS. EGFreceptorsignalingblocksarylhydrocarbonreceptor-mediatedtranscriptionandcelldifferentiationinhumanepidermalkeratinocytes.ProceedingsofNationalAcademyofSciences(2009);106(11):4266-71.
4)HumanPapillomaviruses(HPV)mediatedskincancerresponse:Aninfectionoftheskinwithhumanpapillomaviruses(HPV)mayresultinbenigntumorswithlimitedgrowth,whichtendtoregressspontaneouslyandcausesnon-melanomaskincancerdevelopmentinepidermodysplasiaverruciformis(EV)andimmunosuppressedpatients. ThepresentstudyutilizedpooledkeratinocytesculturedinKGMTM-GoldMediumfromseveralhealthydonors.ThedatashowedthatbetaPVmayincreasethenumberofstemcell-likecellspresentduringearlycarcinogenesisandthusenablethepersistenceandaccumulationofDNAdamagenecessarytogeneratemalignantstemcells. MartinH,AdrianB,CinziaB,MarisaG,JohnD,AlanS,HerbertP,IanMandBakiA.Expressionofbetapapillomavirusoncogenesincreasesthenumberofkeratinocyteswithstemcell-likeproperties.JournalofVirology(2013);87(22):12158-12165.
New!PurchaseourAdultKeratinocyteCellCultureKit(catalogno.192627B)andgetadiscountedpriceoncellsandmedia.Thekitconsistsofacryopreservedampuleofadultkeratinocytes(catalogno.192627)andKGM™-GoldBulletKit™GrowthMedia(catalogno.192060)
