BonemarrowCD34+cellscontainhematopoieticstemandProgenitorcellsandareknowntodifferentiateintoallthevariousbloodcelltypes.
PurityandViABIlity YouCanCountOn
LonzabonemarrowCD34+cellsareisolatedfrombonemarrowmononuclearcells(MNC)bypositiveimmunomagneticseparationandareguaranteedtohaveapurityof≥90%.Theyareguaranteedtocontaineither≥100,000,≥300,000,≥500,000,≥1million,or≥2millionviablecellsperampouledependingontheproductordered.HIV-1,hepatitisC,andhepatitisBarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
FreshbonemarrowCD34+cellsare areofferedinvariouscellspervialpackagingsizesandrequireaminimumorderquantityoftwovialsperdonor.PleasecontactCustomerServiceforinformationonsizesandpricingforfreshcells.
SupportedbyanOptimizedCultureSystem
HPGM™HematopoieticGrowthMediumisrecommendedforoptimalperformancein serum-freeculturing(additionalcytokinesmaybenecessarydependingonapplication)Alternativemediacontainingserummayalsobeacceptable.PleasecontactScientificSupportforserumcontainingmediarecommendations.
ChoicesinCellTypeandTissueSource
Complexresearchoftentimescallsforflexibility. That"swhyweofferisolatedandpurified bonemarrowCD34+cellsaswellas unprocessedbonemarrowincaseyouneed toisolateyourowncells.BonemarrowCD34+cellsare idealforstudiesrequiringalargenumberofcellsfromasingledonororstudiesinvestigatingdifferencesincellsofdonorsofvariousages,genders,orethnicities.Ifhowever,yourstudies demandmorenaïvecells, cordbloodCD34+cellsmaybethebetterchoice.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.
