HumanperipheralbloodCD14+monocytesdifferentiatingtomacrophages
Monocytesarefoundinthecirculatingperipheralbloodwheretheymakeup10-20%ofthetotalmononuclearcellpopulation.MonocytesarecharacterizedbytheirphenotypicexpressionofCD14,andthus,arecommonlycalledCD14+monocytes.Theyplayanimportantroleinhostdefenseascirculatingmonocytesanddifferentiationintotissuemacrophages,andcandifferentiateintodendriticcellswithpotentantigen-presentingcapABIlity.
CellsYouCanCountontoPerform
LonzaHumanPeripheralBloodCD14+Monocytesareisolatedfromperipheralbloodofscreened,healthy,untreated(non-mobilizedperipheralblood)donors.Briefly,mononuclearcells(MNCs)areisolatedfromapheresisderivedperipheralblood.TheMNCpopulationisthenfurtherpurifiedviapositiveimmunomagneticselectiondirectedagainstCD14.
CryopreservedCD14+MonocytesareguaranteedtoexpressCD14andwillcontaineither≥10,20,or40millionviablecellsperampouledependingupontheproductordered.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
ChoicesinCellTypeandTissueSource
Hematopoieticandimmuneresearchrequiresavarietyofdonorsandcelltypes.That"swhyLonzaoffersmanydifferentperipheralbloodcelltypesfromavarietyofdonors,includingPBMCsanddendriticcells,amongothers.Inaddition,ifyouareinterestedinexploringthedifferencesbetweenmonocytesindifferentmaturationstatesorfromdifferenttissues,weofferbonemarrowmononuclearcellsandcordbloodmononuclearcells.
SupportedbyLonzaMedia
Therecommendedmediumvariesbyapplication.PleasecontactScientificSupportforadditionalculturingrecommendations.
- ToMaintaintheMonocytePhenotype:
RPMI+10%FetalBovineSerum +2mM L-glutamine+10ng/mlrHUM-CSF(optional) - ToProduceMacrophages:
RPMI+10%FetalBovineSerum+2mML-glutamine+1%SodiumPyruvate+1%Non-EssentialAminoAcids+25ng/mlrHUM-CSF - ToProduceDendriticCells:
LGM-3™LymphocyteGrowthMedium+50ng/mlGM-CSF+50ng/mlIL-4
Mediarecommendationsformacrophageanddendriticcelldifferentiationarerecommendationsonly.LonzaCD14+monocytesarenotroutinelyqualitycontroltestedformacrophageordendriticcelldifferentiationanddifferentiationisnotguaranteedunderthecellwarranty.Additionalfactorsincludingextracellularmatrixesorculturingconditionsmayberequiredtoproducedifferentiation.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.
