HumanNaturalKiller(NK)Cell
NaturalKiller(NK)cellsarelymphocytesoftheimmunesystemthatarecriticalinhostdefenseandimmuneregulationaspartofboththeinnateandadaptiveimmuneresponse.Theyareofparticularinteresttoimmunotherapyresearchersastheyholdthepromisetospecificallytargetanddestroycancercellsandtumorswhileleavinghealthycellsintact.
CellsYouCanCountontoPerform
NaturalKillercellsaretrADItionallycharacterizedbythepresenceoftheCD56cellsurfaceMarkerandabsenceofCD3.Inaddition,CD16isimportantforNKcellfunctionbecauseCD16inducescytokineexpressionandcytotoxiceffectoractivity.
LonzaNKcellsareisolatedusingpositive(2W-502)ornegative(2W-501)immunomagneticselectionfortheCD56marker.Purityis≥90%basedonthepresenceofCD56+cells.
BothpositivelyandnegativelyselectedNKcellsareguaranteedtoexpressCD56andCD16.However,negativeimmunomagneticseparationofNKcellsresultsinsignificantlygreaterenrichmentofCD16+cells(typically>80%oftotalcells),whereasexpressionofCD16isvariablewiththepositivelyselectedNKcells.
| CatalogNumber | ImmunomagneticSeparation | CD56Expression | CD16Expression |
| 2W-501 | NegativeSelection | ≥90%positive | Typically >80%positive |
| 2W-502 | PositiveSelection | ≥90%positive | Variesbydonor |
LonzaNKcellsareguaranteedtoexpressCD56andCD16,andcontain≥5millionviablecellspervial.HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
ChoicesinCellTypeandTissueSource
ExpressionofCD56andCD16cangreatlyinfluencecellfunction.Inaddition,selectiontechniquescanalsoinfluencefunctionofyourtargetcells.That"swhyLonzaoffersbothpositivelyandnegativelyselectedNKcellsandcharacterizestheexpressionlevelsofbothCD56andCD16ineverylotofcells.
Wealsoofferchoicesintissuesourceifperipheralbloodderivednaturalkillercellsaren"twhatyou"relookingfor.CordbloodMNCsorbonemarrowMNCscanbedepletedoflineagemarkerexpressingcellsinordertoisolateNKcells.Inaddition,cordbloodCD34+cellsorbonemarrowCD34+cellscanbedifferentiatedintonaturalkillercells.
SupportedbyLonzaMedium
LGM-3™LymphocyteGrowthMediumisrecommendedforoptimalperformanceofNKcells.Additionalcytokinesmaybenecessarydependingonapplication.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.
