ProductOverview:
Poietics™NormalHumanDendriticCells are testedforapanelofspecificsurfaceMarkers,including CD11c+,CD86+,CD80+.HLADR+,andCD14-to providedetailed characterizationofthecells.Thesecellscansurviveupto7daysinculturewiththepropercytokines(IL-4andGM-CSF)tomaintainthecellsasimmaturedendriticcells(iDCs).Cellscanbefurther differentiatedtomaturedendriticcellswiththeadditionofTNF-αandIL-1β.Alldendriticcellsaredifferentiatedfromperipheralbloodmonocytes isolatedviaFicolldensitygrADIentseparationandarequalitytestedforoptimalperformance.Lonza"sNormalHumanDendriticCellswouldtypicallybeclassifiedasMo-DC/MDDC(monocytederiveddendriticcells)orCD11c+myeloidDendriticCells(DC).
Applications:
- Antigenpresentationanduptake
- PrimaryResearch
- Pharmacology
- Drug-inducedimmunosuppressionofcytokinesecretion
CryopreservedPoietics™NormalHumanDendriticCells(NHDC)areguaranteedtocontain≥3millionviablecellsperampoule.Expressionlevelsaretestedfor CD80,CD86,HLA-DR,CD11c, andCD14andprovidedforinformationonly. HIV-1,hepatitisBandhepatitisCarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
RecommendedMedium: LGM-3™LymphocyteGrowthMedium+50ng/mlGM-CSF+50ng/mlIL-4
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.
