Cordblood(commonlyabbreviatedHUCB)CD34+cellsareknowntocontainhematopoieticstemandProgenitorcells,andareidealforstudiesrelatingtotransplantationandstemcellsascordbloodderivedcellstendtobemorenaïvethansimilarcellsderivedfromothertissuesources.
HighPurityandViABIlity
LonzacordbloodCD34+cellsareisolatedfromcordbloodmononuclearcells(MNCs)viapositiveimmunomagneticseparationandareguaranteedtohaveapurityof≥90%asindicatedbyCD34+expressingcells.Theyare guaranteedtocontaineither≥100,000,≥500,000,or≥1millionviablecellsperampouledependingontheproductordered.HIV-1andhepatitisBarenotdetectedforalldonorsand/orcelllots.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
Budgetcellsguaranteedtohaveapurityof80-89%asindicatedbyCD34+expressingcellsarealsoavailableatadiscountedratein≥500,000(2C-101AS)or≥1million(2C-101S)viablecellssizes.Contact CustomerServicefororderinginformation.
ChoicesinTissueSource
CordbloodCD34+cellstendtobemorenaivethanhematopoieticstemcellsfromothertissuesources.Thus,theycanbeagreattoolwhentryingtobypassHLAmismatchissues.However,thenumberofcellsavailableperdonorislimitedandcanposeachallengeinyourresearch.Ifyouarelookingforlargenumbersofhematopoieticstemcellsfromthesamedonor,CD34+cellsderivedfrombonemarrowmightbeagoodoption.
SupportedbyanOptimizedMediaSystem
HPGM™HematopoieticGrowthMedium isrecommendedforoptimalperformanceinserum-freeculturing(additionalcytokinesmaybenecessarydependingonapplication).Alternativemediacontainingserummayalsobeacceptable.PleasecontactScientificSupportforserumcontainingmediarecommendations.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.
