Cordbloodmononuclearcells(MNC)arebloodcellscontainingaroundednucleus(includingmacrophages,monocytes,andlymphocytes).Cordbloodhematopoieticstemcells(HSC)andhematopoieticProgenitorcells(HPC)canbereADIlyisolatedfromthemononuclearcell(MNC)fraction.
CyropreservedPoetics™CordBloodMononuclearCells(MNC)areisolatedfromwholecordbloodviadensitygradientcentrifugationandareguaranteedtocontaineither≥50million(2C-150B),≥100million(2C-150A),or≥200million(2C-150)viablecellsperampoule.
Alldonorsand/orcelllotstestnegativeforHIV-1,hepatitisBandhepatitisC.ACertificateofAnalysisisprovidedforeachcelllotpurchased.
RecommendedMedium:HPGM™HematopoieticGrowthMedium forserum-freeculturing(additionalcytokinesmaybenecessarydependingonapplication).Alternativemediacontainingserummayalsobeacceptable.PleasecontactScientificSupportforserumcontainingmediarecommendations.
LonzaguaranteestheperformanceofClonetics™/Poietics™cellsonlyifappropriateClonetics™/Poietics™mediaandreagentsareusedexclusivelyandtherecommendedstorageanduseprotocolsarefollowed.Anymodificationsmadetotherecommendedcellsystemsincludingtheuseofalternativemedia,reagentsorprotocols,willvoidcellandmediaperformanceguarantees.Ifyouneedassistanceinselectingtheappropriatemedia,reagents,orprotocol,pleasecontactLonzaScientificSupport.

